Healthy cloned monkeys born in Shanghai
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Thursday, January 25, 2018
In findings published Wednesday in the scientific journal Cell, a team of scientists from the Chinese Academy of Sciences in Shanghai, China have announced the first-ever cloning of a primate from post-embryonic cells, namely two macaque monkeys. They used somatic cell nuclear transfer, the same method that was used to create Dolly the sheep in 1996.
In somatic cell nuclear transfer, scientists remove the nucleus, which is the organelle that contains the chromosomes, from an unfertilized ovum, or egg cell, and implant the nucleus from a somatic cell, or non-reproductive cell, into that ovum. The ovum is then stimulated and develops in the normal way, growing into a whole organism that has the same nuclear DNA as the donor organism, though it will have all of the ovum’s mitochondria and other cellular machinery. Clones like these have been described as identical twins to their donors, but younger.
The scientists implanted 21 ova into surrogate mother monkeys, resulting in six pregnancies, two of which produced living animals. The young clones were named “Zhong Zhong” and “Hua Hua,” both derived from Zh?nghuá, the Chinese-language word for the Chinese people. They are both cynomolgus monkeys, or crab-eating macaques (Macaca fascicularis). The scientists also attempted to clone macaques using nuclei from adult donors. They implanted 42 surrogates, resulting in 22 pregnancies, but there were still only two infant macaques, and they died soon after birth. The Scotland-based team that created Dolly the sheep in 1996 required 277 attempts and produced only one lamb.
Generally, the older the donor organism, the more difficult it is to get the DNA from the harvested nucleus to reactivate the genes that allow the clone organism to grow. Previous efforts to clone rhesus monkeys (Macaca mulatta) using embryonic donor cells have been successful, but the current attempt used significantly older donors: fetal fibroblast cells, which are key cells in connective tissue, and adult monkey cumulus cells.
“We’re excited — extremely excited,” said study co-author Muming Poo. “This is really, I think, a breakthrough for biomedicine.” He went on to say that the cloned monkeys could be used as test subjects for the study of neurological diseases such as Parkinson’s and Huntington’s. Primates are already a popular model organism for neurological studies. In the United States, for example, non-human primates are used in less than 0.3% of all animal experiments, most of them involving neuroscience, and macaques in particular are a well-established animal model of atherosclerosis, which causes heart disease.
“I think it’s a very exciting landmark. It’s a major advance,” agreed reproductive biologist Dieter Egli of Columbia University. “It should be possible to make models of human disease in those monkeys and study those and then attempt to cure it.”
About 90% of the laboratory animals used in the United States are rodents. Although the first cloned mouse was born in 1998, cloned mice are not currently common in laboratory settings. This may be because producing inbred mouse lines is still relatively effective.
Although the announcement raised enthusiasm from researchers, it also drew caution from bioethicists. “Cloning one individual in the image of another really sort of demeans the significance of us as individuals,” says Harvard Medical School’s Dr. George Daley, speaking specifically of cloning humans. “There’s a certain sort of gut sense that it violates sort of natural norms.” While Muming Poo concedes it is now theoretically possible to clone a human, he says his lab has no plans to do so.
Although somatic cell nuclear transfer was used successfully in amphibians as early as 1952, getting it to work in mammals took much longer. Dolly, the first cloned mammal, was born in 1996. Teams have been trying to clone monkeys for decades, but primate DNA is notoriously difficult to work with. “The trick is we choose the right chemicals to turn on these genes we transfer into the egg,” Mu-ming Poo told the press. “So that’s what we did different. I think that’s the key.” One of the agents used to treat the ova was messenger RNA from the human Kdm4d gene.
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Veteran U.S. attorney appointed to oversee Plame inquiry
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Saturday, August 13, 2005
U.S. Dept. of Justice attorney for 40 years, Dave Margolis, was assigned by James Comey on August 12 to take over his position as the acting Deputy Attorney General. The former assistant deputy replaces Comey as the acting boss of Patrick Fitzgerald, the lead prosecutor of the Plame CIA leak case. Comey left the post for the private sector as general council for Lockheed Martin Corp.
Al Martin, ret. Navy Lt. Commander (who testified for Kerry’s Iran-Contra hearings) alleges that when David Margolis was the Domestic Criminal Section Chief, he helped limit the liability of Ronald Reagan and George H. W. Bush in the Iran-Contra affair. Martin is also on record as having alleged that Margolis was part of a “restricted access group” known as RAG-1, which “was first to develop and then to coordinate the CIA’s policy of trafficking in narcotics on a large-scale basis, in order to produce ongoing covert revenue streams pursuant to the aid and sustenance of illegal operations of state,” according to Martin.
President Bush nominated Timothy E. Flanigan for Comey’s job. The Comey departure, without Margolis stepping in, would have left two key posts at the Justice Department unfilled. The head of the Criminal Justice, formerly occupied by Christopher Wray, has been empty since May.
Flanigan is facing tough questioning during his Senate Judiciary Committee confirmation process. Senator Arlen Specter (R-PA) said he is unsure whether he would vote for his confirmation. Flanigan’s role in the aggressive interrogation techniques used on detainees from Afghanistan and Iraq and his ties to lobbyist Jack Abramoff are being probed.
